A Practical Handbook of Preparative HPLCElsevier, 2011. gada 18. apr. - 176 lappuses This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.This book...•Provides practical, hands-on advice based on years of experience•Will help ensure optimal design, equipment and separation results for your particular task•Presents system layouts from laboratory to process scale•Will help you to devise or improve record-keeping and documentation systems ·Provides practical, hands-on advice based on years of experience·Will help ensure optimal design, equipment and separation results for your particular task·Presents system layouts from laboratory to process scale·Will help you to devise or improve record-keeping and documentation systems |
No grāmatas satura
1.–5. rezultāts no 7.
xi. lappuse
... theoretical plate HPLC high performance liquid chromatography IQ installation qualification IUPAC International Union for Pure and Applied Chemists MSDSs material safety data sheets OQ operational qualification PQ performance ...
... theoretical plate HPLC high performance liquid chromatography IQ installation qualification IUPAC International Union for Pure and Applied Chemists MSDSs material safety data sheets OQ operational qualification PQ performance ...
20. lappuse
... theoretical plates' is used to define the efficiency of the packed column bed. The mathematical derivations for plate theory were initially developed by Martin and Synge[2.3] and published in 1941. Why plates? This term actually ...
... theoretical plates' is used to define the efficiency of the packed column bed. The mathematical derivations for plate theory were initially developed by Martin and Synge[2.3] and published in 1941. Why plates? This term actually ...
21. lappuse
... theoretical plates, N and the separation factor, . 1 1/2 2 ⎛ ⎝ ⎜ ⎜ ⎜ ⎜ ⎞ ⎠ ⎟ ⎟ ⎟ ⎟⎟ N 5.54 V W The separation factor , is effectively a measure of the. Figure 2.2 shows the measurements to be taken from a typical the most ...
... theoretical plates, N and the separation factor, . 1 1/2 2 ⎛ ⎝ ⎜ ⎜ ⎜ ⎜ ⎞ ⎠ ⎟ ⎟ ⎟ ⎟⎟ N 5.54 V W The separation factor , is effectively a measure of the. Figure 2.2 shows the measurements to be taken from a typical the most ...
22. lappuse
... theoretical plate, HETP, which is simply the length of the column divided by the number of theoretical plates, N. The path of a molecule dissolved in the solvent passing through a packed bed is fraught with obstacles. This individual ...
... theoretical plate, HETP, which is simply the length of the column divided by the number of theoretical plates, N. The path of a molecule dissolved in the solvent passing through a packed bed is fraught with obstacles. This individual ...
88. lappuse
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Esat sasniedzis šīs grāmatas aplūkošanas reižu limitu.
Saturs
17 | |
29 | |
4 How to get started | 57 |
5 Process development and optimization | 77 |
6 Documentation and record keeping | 101 |
back matter | 111 |
References | 159 |
Index | 167 |
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__________ Date __________ __________ Operator __________ acetonitrile affinity chromatography amino acids analytical column Analytical HPLC anion exchange ation band broadening Batch number chemical chiral chromatography chiral stationary phases chro chromatog cm3/min Column diameter column loading column packing column walls concentration DAC column desorbed diameter column document effect efficient eluent enantiomers equipment filter flask fume cupboard high pH high pressure HPLC system HPLC traces hydrophilic impurities ion exchange Isocratic main component manufacturers mass transfer microfibre mixture mobile phase mode of chromatography mode of separation molecule normal phase chromatography Number Number number of Buffer oligonucleotides Operator __________ Date packed bed particles peptide pharmaceutical polymeric stationary phases pool __________ pool meets pore preparative HPLC preparative separation process instruction proteins PURIFICATION NUMBER Record the following reversed phase chromatography reversed phase media scale slurry solvent stationary phase surface technique theoretical plates tion tionary phase trial pool trifluoroacetic acid typical volume yes/no