Enzyme Immunoassays: From Concept to Product DevelopmentSpringer Science & Business Media, 1996. gada 30. jūn. - 464 lappuses This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market. |
Saturs
Introduction | 3 |
A Historical Perspective | 8 |
Diagnostic Markets and Economics | 12 |
Scope of the Book | 19 |
References | 21 |
Antibodies Biochemistry Structure and Function | 24 |
Antibody Classification | 32 |
Antibody Structure | 36 |
References | 224 |
Product Development | 229 |
Immunoassay Classification and Commercial Technologies | 231 |
Classification | 232 |
Heterogeneous Enzyme Immunoassays | 240 |
Homogeneous Enzyme Immunoassays | 244 |
Commercial Technologies | 258 |
References | 269 |
Antibody Functions | 40 |
Auxiliary Binding Systems | 41 |
References | 48 |
AntigenAntibody Reactions | 52 |
AntigenAntibody Binding | 53 |
Antibody Affinity and Avidity | 57 |
antibody Specificity and CrossReactivity | 59 |
Quantitation of AntigenAntibody Reactions | 60 |
References | 70 |
Conjugation Techniques | 72 |
Functional Groups on Proteins | 73 |
Chemical Modification of Protein Functional Groups | 76 |
CrossLinking Reagents for Protein Conjugation | 78 |
Practical Aspects | 104 |
References | 110 |
Antibody Production | 117 |
Nature and Preparation of Immunogen | 119 |
Immunization | 133 |
Antibody Screening | 138 |
Monoclonal Antibodies | 140 |
Purification | 144 |
Stabilization and Storage | 149 |
References | 151 |
Enzymes and Signal Amplification Systems | 155 |
Enzymes | 162 |
Signal Amplification Systems | 182 |
References | 187 |
Separation and SolidPhase Systems | 193 |
SolidPhase Systems | 206 |
Immobilization of Immunoreagents | 216 |
Assay Development Evaluation and Validation | 275 |
Selection of Assay Format | 277 |
Preparation of the Solid Phase | 283 |
Blocking | 302 |
Enzyme Labels | 304 |
Sample and Standard Preparation | 305 |
Assay Conditions | 307 |
Assay Evaluation | 313 |
Quality AssuranceQuality Control QAQC | 341 |
Assay Validation | 343 |
References | 353 |
Reagent Formulations and Shelf Life Evaluation | 360 |
Liquid Formulations | 361 |
Solid Formulations | 366 |
Preparation of Reference Standards and Calibrators | 381 |
Shelf Life Evaluation | 386 |
References | 397 |
Data Analysis | 401 |
Standard or Calibration Curves | 402 |
CurveFitting Techniques | 409 |
Statistical Descriptors | 415 |
References | 423 |
Documentation Registration and Diagnostic StartUps | 426 |
Documentation Systems | 427 |
ISO 9000 Certification | 435 |
ISO 9000 Series Standards | 437 |
Diagnostics StartUps | 445 |
| 450 | |
| 453 | |
Citi izdevumi - Skatīt visu
Enzyme Immunoassays: From Concept to Product Development S.S. Deshpande Ierobežota priekšskatīšana - 2012 |
Enzyme Immunoassays: From Concept to Product Development S.S. Deshpande Priekšskatījums nav pieejams - 2011 |
Bieži izmantoti vārdi un frāzes
acid adsorbed adsorption affinity alkaline phosphatase amino groups analyte concentration anti antigen antigen-antibody antiserum application assay sensitivity automated avidin binding sites Biochem bound buffer calibration curve capture antibody carboxyl cells Chem chemical Clin clinical coating competitive complex compounds conjugate coupling covalent cross-linking cross-reactivity described detection determined diagnostic dilution drug effect Ekins enzyme immunoassays enzyme label epitopes evaluation Figure fraction functional hapten homogeneous hormones hydrophobic immobilized immune immunoassay system immunodiagnostic immunogen immunoglobulin immunometric increased incubation laboratory ligand limited matrix measurement membrane methods microtiter plates molecules monoclonal antibodies nonspecific optimal particles performance polyclonal polyclonal antibodies polystyrene precision preparation primary procedures protein quality system quantitative radioimmunoassay range reaction reactive reagents receptor response sample sensitivity separation serum shelf solid phase solution specific stability standard substrate surface techniques temperature theophylline thiol Tijssen tion tubes