alpha numeric field-rep, to a maximum of ten classification designations):

(vii) PRIOR APPLICATION DATA (prior domestic, foreign priority or international application data, if applicable-M/rep):

(A) APPLICATION NUMBER (application number; specify as two letter country code and an eight digit application number; or if a PCT application, specify as the letters PCT, a slash, a two letter code indicating the Receiving Office, a two digit indication of the year, a slash and a five digit number):

(B) FILING DATE (document filing date, specify as dd-MMM-yyyy):

(viii) ATTORNEY/AGENT INFORMATION (0):

(A) NAME (attorney/agent name; SURNAME comma OTHER NAMES and/or INITIALS):

(B) REGISTRATION NUMBER (attorney/agent registration number):

(C) REFERENCE/DOCKET NUMBER (attorney/agent reference or docket number):

(ix) TELECOMMUNICATION INFORMATION (0):

(A) TELEPHONE (telephone number of applicant or attorney/agent):

(B) TELEFAX (telefax number of applicant or attorney/agent):

(C) TELEX (telex number of applicant or attorney/agent):

(2) INFORMATION FOR SEQ ID NO: X(rep):

(1) SEQUENCE CHARACTERISTICS (M):

(A) LENGTH (sequence length, expressed as number of base pairs or amino acid residues):

(B) TYPE (sequence type, i.e., whether nucleic acid or amino acid):

(C) STRANDEDNESS (if nucleic acid, number of strands of source organism molecule, i.e., whether single stranded, double stranded, both or unknown to applicant):

(D) TOPOLOGY (whether source organism molecule is circular, linear, both or unknown to applicant)

(ii) MOLECULE TYPE (type of molecule sequenced in SEQ ID NO:X (at least one of the following should be included with subheadings, if any, in Sequence Listing-R)):

-Genomic RNA; -Genomic DNA;

-mRNA -tRNA; -rRNA; -snRNA; -SCRNA; -preRNA;

-CDNA to genomic RNA; -CDNA to mRNA; -CDNA to tRNA;

CDNA to rRNA; -CDNA to snRNA; -CDNA to scRNA; -Other nucleic acid;

(A) DESCRIPTION (four lines maximum):

-protein and -peptide.

(iii) HYPOTHETICAL (yes/no-R): (iv) ANTI-SENSE (yes/no-R):

(v) FRAGMENT TYPE (for proteins and peptides only, at least one of the following should be included in the Sequence Listing-R):

-N-terminal fragment;

-C-terminal fragment and -internal fragment.

(vi) ORIGINAL SOURCE (original source of molecule sequenced in SEQ ID NO:X-R):

(A) ORGANISM (scientific name of source organism):

(B) STRAIN:

(C) INDIVIDUAL ISOLATE (name/ number of individual/isolate):

(D) DEVELOPMENTAL STAGE (give developmental stage of source organism and indicate whether derived from germ-line or rearranged developmental pattern):

(E) HAPLOTYPE: (F) TISSUE TYPE: (G) CELL TYPE: (H) CELL LINE: (I) ORGANELLE:

(vii) IMMEDIATE SOURCE (immediate experimental source of the sequence in SEQ ID NO:X-R):

(A) LIBRARY (library-type, name): (B) CLONE (clone(s)):

(viii) POSITION IN GENOME (position of sequence in SEQ ID NO:X in genome-R):

(A) CHROMOSOME/SEGMENT (chromosome/segment-name/number): (B) MAP POSITION:

(C) UNITS (units for map position, i.e., whether units are genome percent, nucleotide number or other/specify):

(D) VOLUME (journal volume in which data published):

(E) ISSUE (journal issue number in which data published):

(F) PAGES (journal page numbers in which data published):

(G) DATE (journal date in which data published; specify as dd-MMM-yyyy, MMM-yyyy or Season-yyyy):

(H) DOCUMENT NUMBER (document number, for patent type citations only; specify as two letter country code, eight digit document number (right justified), one letter and, as appropriate, one number or a space as a document type code; of if a PCT application, specify as the letters PCT, a slash, a two letter code indicating the Receiving Office, a two digit indication of the year, a slash and a five digit

number; or if a PCT publication, specify as the two letters WO, a two digit indication of the year, a slash and a five digit publication number):

(I) FILING DATE (document filing date, for patent-type citations only; specify as dd-MMM-yyyy):

(J) PUBLICATION DATE (document publication date; for patent-type citations only, specify as dd-MMM-yyyy):

(K) RELEVANT RESIDUES In SEQ ID NO:X (rep): FROM (position) TO (position)

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:X:

$1.824 Form and format for nucleotide and/or amino acid sequence submissions in computer readable form. (a) The computer readable form required by §1.821(e) shall contain a printable copy of the "Sequence Listing," as defined in §§ 1.821(c), 1.822 and 1.823, recorded as a single file on either a diskette or a magnetic tape. The computer readable form shall be encoded and formatted such that a printed copy of the "Sequence Listing" may be recreated using the print commands of the computer/operating-system configuration specified in paragraph (f) of this section.

(b) The file in paragraph (a) of this section shall be encoded in a subset of the American Standard Code for Information Interchange (ASCII). This subset shall consist of all the printable ASCII characters including the ASCII space character plus line-termination, pagination and end-of-file characters associated with the computer/operating-system configurations specified in paragraph (f) of this section. No other characters shall be allowed.

(c) The computer readable form may be created by any means, such as word processors, nucleotide/amino acid sequence editors or other custom computer programs; however, it shall be readable by one of the computer/operating-system configurations specified in paragraph (f) of this section, and shall conform to the specifications in paragraphs (a) and (b) of this section.

(d) The entire printable copy of the "Sequence Listing" shall be contained within one file on a single diskette or magnetic tape unless it is shown to the satisfaction of the Commissioner that

it is not practical or possible to submit the entire printable copy of the "Sequence Listing" within one file on a single diskette or magnetic tape.

(e) The submitted diskette or tape shall be write-protected such as by covering or uncovering diskette holes, removing diskette write tabs or removing tape write rings.

(f) As set forth in paragraph (c), above, any means may be used to create the computer readable form, as long as the following conditions are satisfied. A submitted diskette shall be readable on one of the computer/operating-system configurations described in paragraphs (1) through (3), below. A submitted tape shall satisfy the format specifications described in paragraph (4), below.

(1) Computer: IBM PC/XT/AT, IBM PS/2 or compatibles;

(1) Operating system: PC-DOS or MSDOS (Versions 2.1 or above);

(ii) Line Terminator: ASCII Carriage Return plus ASCII Line Feed;

(iii) Pagination: ASCII Form Feed or Series of Line Terminators;

(iv) End-of-File: ASCII SUB (Ctrl-Z); (v) Media: (A) Diskette-5.25 inch, 360 Kb storage;

(B) Diskette 5.25 inch, 1.2 Mb storage;

(C) Diskette 3.50 inch, 720 Kb storage;

(D) Diskette 3.5 inch, 1.44 Mb storage;

Command: PRINT

(vi) Print filename.extension;

(2) Computer: IBM PC/XT/AT, IBM PS/2 or compatibles;

(i) Operating system: Xenix;

(ii) Line Terminator: ASCII Carriage Return;

(iii) Pagination: ASCII Form Feed or Series of Line Terminators;

(iv) End-of-File: None;

(v) Media: (A) Diskette—5.25 inch, 360 Kb storage;

(B) Diskette 5.25 inch, 1.2 Mb storage;

(C) Diskette-3.50 inch, 720 Kb storage;

(D) Diskette-3.5 inch, 1.44 Mb storage;

(vi) Print Command: Ipr filename;
(3) Computer: Apple Macintosh;
(i) Operating System: Macintosh;

(ii) Macintosh File Type: text with line termination

(iii) Line Terminator: Pre-defined by text type file;

(iv) Pagination: Pre-defined by text type file;

(v) End-of-file: Pre-defined by text type file;

(vi) Media: (A) Diskett-3.50 inch, 400 Kb storage;

(B) Diskette 3.50 inch, 800 Kb storage;

(C) Diskette 3.50 inch, 1.4 Mb storage;

(vii) Print Command: Use PRINT command from any Macintosh Application that processes text files, such as MacWrite or Teach Text;

(4) Magnetic tape: 0.5 inch, up to 2400 feet;

(i) Density: 1600 or 6250 bits per inch, 9 track;

(ii) Format: raw, unblocked;

(iii) Line Terminator: ASCII Carriage Return plus optional ASCII Line Feed; (iv) Pagination: ASCII Form Feed or Series of Line Terminators;

(v) Print Command (Unix shell version given here as sample response— mt/dev/rmt0; 1pr/dev/rmt0):

(g) Computer readable forms that are submitted to the Office will not be returned to the applicant.

(h) All computer readable forms shall have a label permanently affixed thereto on which has been hand printed or typed, a description of the format of the computer readable form as well as the name of the applicant, the title of the invention, the date on which the data were recorded on the computer readable form and the name and type of computer and operating system which generated the files on the computer readable form. If all of this information cannot be printed on a label affixed to the computer readable form, by reason of size or otherwise, the label shall include the name of the applicant and the title of the invention and a reference number, and the additional information may be provided on a container for the computer readable form with the name of the applicant, the title of the invention, the reference number and the additional information affixed to the container. If the computer readable form is submitted after the date of filing under 35 U.S.C. 111,

after the date of entry in the national stage under 35 U.S.C. 371 or after the time of filing, in the United States Receiving Office, an international application under the PCT, the labels mentioned herein must also include the date of the application and the application number, including series code and serial number.

§1.825 Amendments to or replacement of sequence listing and computer readable copy thereof.

(a) Any amendment to the paper copy of the “Sequence Listing” (§1.821(c)) must be made by the submission of substitute sheets. Amendments must be accompanied by a statement that indicates support for the amendment in the application, as filed, and a statement that the substitute sheets include no new matter. Such a statement must be a verified statement if made by a person not registered to practice before the Office.

(b) Any amendment to the paper copy of the "Sequence Listing," in accordance with paragraph (a) of this section, must be accompanied by a substitute copy of the computer readable form (§1.821(e)) including all previously submitted data with the amendment incorporated therein, accompanied by a statement that the copy in computer readable form is the same as the substitute copy of the "Sequence Listing." Such a statement must be a verified statement if made by a person not registered to practice before the Office.

(c) Any appropriate amendments to the "Sequence Listing" in a patent, e.g., by reason of reissue or certificate of correction, must comply with the requirements of paragraphs (a) and (b) of this section.

(d) If, upon receipt, the computer readable form is found to be damaged or unreadable, applicant must provide, within such time as set by the Commissioner, a substitute copy of the data in computer readable form accompanied by a statement that the substitute data is identical to that originally filed. Such a statement must be a verified statement if made by a person not registered to practice before the Office.

APPENDIX A TO SUBPART G OF PART 1— SAMPLE SEQUENCE LISTING

(1) GENERAL INFORMATION:

(1) APPLICANT: Doe, Joan X, Doe, John Q
(11) TITLE OF INVENTION: Isolation and
Characterization of a Gene Encoding a
Protease from Paramecium sp.

(iii) NUMBER OF SEQUENCES: 2
(iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: Smith and Jones
(B) STREET: 123 Main Street
(C) CITY: Smalltown
(D) STATE: Anystate
(E) COUNTRY: USA
(F) ZIP: 12345

(v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Diskette, 3.50 inch, 800 Kb storage

(B) COMPUTER: Apple Macintosh

(C) OPERATING SYSTEM: Mcintosh 5.0
(D) SOFTWARE: MacWrite

(vi) CURRENT APPLICATION DATA:
(A) APPLICATION NUMBER: 09/999,999
(B) FILING DATE: 28-FEB-1989
(C) CLASSIFICATION: 999/99

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: PCT/US88/ 99999

(B) FILING DATE: 01-MAR-1988

(viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Smith, John A.

(B) REGISTRATION NUMBER: 00001 (C) REFERENCE/DOCKET NUMBER: 010001

(2) INFORMATION FOR SEQ ID NO: 1: (1) SEQUENCE CHARACTERISTICS: (A) LENGTH: 954 base pairs (B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: genomic DNA
(iii) HYPOTHETICAL: yes
(iv) ANTI-SENSE: no

(vi) ORIGINAL SOURCE:

(A) ORGANISM: Paramecium sp (C) INDIVIDUAL/ISOLATE: XYZ2 (G) CELL TYPE: unicellular organism (vii) IMMEDIATE SOURCE: (A) LIBRARY: genomic

(B) CLONE: Para-XYZ2/36

(x) PUBLICATION INFORMATION: (A) AUTHORS: Doe, Joan X, Doe, John Q (B) TITLE: Isolation and Characterization of a Gene Encoding a Protease from Paramecium sp.

(C) JOURNAL: Fictional Genes (D) VOLUME: I

(E) ISSUE: 1

(F) PAGES: 1-20

(G) DATE: 02-MAR-1988

(K) RELEVANT RESIDUES IN SEQ ID NO: 1: FROM 1 TO 954