Handbook of Biological Confocal MicroscopyJames B. Pawley Springer Science & Business Media, 1995 - 632 lappuses New edition of a reference originally published in 1989 and revised in 1990. The present edition includes 37 chapters (15 updated from the first edition, 21 new ones, and an annotated bibliography), and is nearly three times as long as the original. In particular, it reflects an increased recognitio |
Saturs
Foundations of Confocal Scanned Imaging in Light Microscopy | 1 |
Fundamental Limits in Confocal Microscopy | 19 |
Quantitative Fluorescence Confocal Laser Scanning Microscopy CLSM | 39 |
The Pixilated Image | 55 |
Laser Sources for Confocal Microscopy | 69 |
NonLaser Light Sources | 99 |
Objective Lenses for Confocal Microscopy | 111 |
The Specimen Illumination Path and Its Effect on Image Quality | 127 |
Lens Aberrations in Confocal Fluorescence Microscopy | 347 |
RealTime Stereo 3D Confocal Microscopy | 355 |
SignaltoNoise in Confocal Microscopes | 363 |
Comparison of WideFieldDeconvolution and Confocal Microscopy for 3D Imaging | 373 |
Light Microscopic Images Reconstructed by Maximum Likelihood Deconvolution | 389 |
Direct View Confocal Imaging Systems Using a Slit Aperture | 403 |
Two New HighResolution Confocal Fluorescence Microscopies 4Pi Theta with One and TwoPhoton Excitation | 417 |
Optical Considerations at Ultraviolet Wavelengths in Confocal Microscopy | 431 |
The Intermediate Optical System of LaserScanning Confocal Microscopes | 139 |
Intermediate Optics in Nipkow Disk Microscopes | 155 |
The Role of the Pinhole in Confocal Imaging System | 167 |
Photon Detectors for Confocal Microscopy | 183 |
The Collection Processing and Display of Digital ThreeDimensional Images of Biological Specimens | 197 |
Visualization Systems for Multidimensional CLSM Images | 211 |
Mapping and Measuring Surfaces Using Reflection Confocal Microscopy | 255 |
Fluorophores for Confocal Microscopy | 267 |
Image Contrast in Confocal Light Microscopy | 281 |
Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy | 311 |
Confocal Microscopy of Living Cells | 327 |
TwoPhoton Molecular Excitation in LaserScanning Microscopy | 445 |
VideoRate Confocal Microscopy | 459 |
Confocal Microscopy with Transmitted Light | 479 |
Fluorescence Lifetime Imaging a New Tool in Confocal Microscopy | 491 |
Imaging Immunogold Labels with Confocal Microscopy | 507 |
Fiberoptics in Confocal Microscopy | 515 |
Comparison of Various Optical Sectioning Methods | 525 |
Mass Storage and Hard Copy | 535 |
Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen | 549 |
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